A. 2D-DIGE and immunoblot analysis were employed in a well-defined lung carcinogenesis mouse model to identify potential protein biomarkers of lung cancer. Down-regulation of 14-3-3 isoforms and annexin A5, observed in lung adenocarcinomas from mice treated with the tobacco-specific carcinogen NNK, were subsequently recovered in the lungs of mice treated with the chemopreventive agent p-XSC. The possible involvement of these proteins in cancer-associated pathways supports their potential use as biomarkers to monitor lung carcinogenesis (Bortner JD Jr, Das A, Umstead TM, Freeman WM, Somiari R, Aliaga C, Phelps DS, El-Bayoumy K, J Proteome Res. 2009 Aug;8(8):4050-61).
B. Tobacco smoking is one of the leading causes for oral cancer. We have developed a mice model for oral cancer using environmental carcinogen and tobacco smoke constituent DB[a,l]P. We have also developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect and quantify anti-DB[a,l]PDE-DNA adducts in oral tissues of mice treated with DB[a,l]P. The results indicate that the formation and persistence of DB[a,l]PDE-DNA adducts may, in part, contribute to the initiation of DB[a,l]P-induced oral carcinogenesis (Zhang SM, Chen KM, Aliaga C, Sun YW, Lin JM, Sharma AK, Amin S, El-Bayoumy K, Chem Res Toxicol. 2011 Aug 15;24(8):1297-303).
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